It is critical to purge the air from the tubing of a blood collection set when collecting a sodium citrate tube for PT and/or APTT testing. A plain discard tube or another sodium citrate tube should be used first to purge the air from the tubing and allow a full draw into the citrate tube. Purging the air from the tubing is also recommended before drawing any additive tube.
CLSI recommends that all additive tubes should be filled to their stated volume. More.
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