How can I change a pixel value from a grayscaled image using Opencv 2.3?

Since a grayscale image contains only one component instead of 3, the resulting matrix/image is of type CV_8UC1 instead of CV_8UC3 . And this in turn means, that individual pixels are not 3-vectors of bytes ( cv::Vec3b ) but just single bytes ( unsigned char or OpenCV's uchar ). So you can just use.

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