How does the method differentiate between the cell–cell junctions and the cell–matrix attachments?

The turnover of vinculin in cell–cell adhesions is much more rapid than the turnover in cell–matrix adhesions. When we inhibit ∼70–80% of the vinculin, it leaves intact the vinculin at sites of cell–matrix adhesion, and only removes vinculin's function in cell–cell adhesion. Our results show that when we add the fluorescent version of the protein, we are looking at vinculin at the cell–cell junctions.

So what have you discovered? We're very close to ascribing a function for vinculin at the cell–cell junctions. But we've not published it yet.

In short, what we've found is that vinculin is one of the proteins that appears to be involved in dynamic changes in adhesive properties that occur during processes such as transendothelial migration, mitosis, embryonic development, or wound healing. Vinculin (green) colocalizes with a cell–cell adhesion protein (red). What's up next for you?

First we want to establish vinculin's function, and then we'll pursue how it fits into the broader ... more.

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