How to enable Windows Authentication and NetTCPBinding WCF webservice on IIS7?

The default security setting for NetTCP is Windows Authentication. It sounds like one or two things might be the issue.

In the IIS management console, browse to the site and application where your service is being hosted. Then make sure you have the "Features View" selected, and select the "Authentication" option. You should see various forms of authentication (anonymous, Windows, ASP.NET Impersonation, etc.) and each should say either enabled or disabled next to it.

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