Rewrite your function to accept both conventions.
Rewrite your function to accept both conventions: function = myfun(v1,v2,v3,v4,v5,v6) if nargin==1 v2 = v1(:,2); v3 = v1(:,3); v4 = v1(:,4); v5 = v1(:,5); v6 = v1(:,6); v1 = v1(:,1); end %# use v1,v2,v3,v4,v5,v6 %# ... end Now you can call as both: myfun(A(:,1),A(:,2),A(:,3),A(:,4),A(:,5),A(:,6)) myfun(A) Usually you would do more validation to test for the correct number of arguments...
You can first place each column of A in a cell of a cell array using the function NUM2CELL, then pass the cell array contents as a comma-separated list using the {:} syntax: B = num2cell(A,1); myfun(B{:}).
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