Each time a TCP connection is made, Twisted will create a unique instance of TSServerProtocol to handle that connection. So, you'll only ever see 1 connection in TSServerProtocol . Normally, this is what you want but Factories can be extended to do the connection tracking you're attempting to do here.
Specifically, you can subclass Factory and override the buildProtocol() method to track instances of TSServerProtocol . The interrelationship between all the classes in Twisted takes a little time to learn and get used to. In particular, this piece of the standard Twisted documentation should be your best friend for the next while ;-).
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