Upon screening recombinants with Pac I, some clones give a 30-35 kb band and a 3.0 kb band, others the same 30-35 kb and a 4.5 kb band. Which one is the good pattern?

A. Some recombinants might come from a recombination event between the right side homologous regions and the origins of replication instead of with the left homologous regions (see applications manual). These 2 recombinants are equally good and can very well be used but will give different restriction patterns.

In our own experience, the pattern giving a band at 4.5 kb is generally the most abundant. More.

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