What are the known interferences for the blood spot assay?

€¢ Following is a table summarizing the assay manufacturers claims regarding the effect of interfering steroids on the 17OHP newborn screening assay: • Please click here for a table that summarizes the experimental data we ourselves obtained regarding interfering substances. As you can see there is a small effect (~15%) from a very high concentration of 17OH-pregnenenlone (500nM). There is, however, a marked interference from EDTA so it is important that this anticoagulant is not used in collecting blood for spotting on the dried blood spot card.

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