There are a lot of buildings and sights from the 1935-1936 California Exposition that still exist today, particularly in the Southern end of Balboa Park. The Ford Motor Company had a special building constructed in Balboa Park to showcase their automobiles during the fair. The Ford Building still exists, and currently houses the San Diego Air and Space Museum.
There are two murals in the building that date to the 1936 season of the exposition. One, known as "The March of Transportation", shows a variety of forms of transportation throughout history, as well as showing the artist's conception of possible future methods of transportation. The other mural deals with the National Geographic Explorer II gondola, which had taken a historic, record-breaking flight in 1935.
The Spanish Village Art Center is currently housed in buildings that were built to represent a Spanish village during the Exposition. You can see the works of over 200 artists and craftspeople at the SVAC. It is open 7 days a week, closed only for Thanksgiving, Christmas, and New Years.
A replica of the Globe Theatre was built for the Exposition. The theatre has been destroyed by fire twice since then. You can see it in its current incarnation in the Old Globe Theatre Complex, along with the Cassius Carter Centre Stage and the Lowell Davies Festival Stage.
http://www.theoldglobe.org/index.aspx The Palisades Building was originally built for the exposition. In 1935 it was known as the Women's Palace. In 1936 it was called the Palace of Entertainment.It currently houses the Marie as well as a recital hall and a division of the Park & Recreation Department.
In fact, many of the buildings in the Palisades area originated during the second Exhibition, including the Municipal Gymnasium, the House of Pacific Relations, the Ford Building (mentioned above), the California State Building (currently housing the San Diego Automotive Museum), the Federal Building (currently housing the San Diego Hall of Champions Sports Museum), and the Starlight Bowl. Zoro Gardens (the site of a "nudist colony" exhibit during the exposition) and the Alcazar garden still exist. The Alcazar garden is currently a replica of its 1935 design.
While I am not aware of tours specifically for the second exposition, there are several tours dealing with the history of Balboa park that would probably cover the exposition and its legacy in the park. You can read about them here: http://www.balboapark.org/info/tours.php In addition, many of the buildings that still exist have their own tours that would certainly touch on the second Exhibition.
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