What can I do to avoid noise during sectioning (knife produces a screeching noise) and/or sections with chatter marks or scratches?

The sectioning speed is too high: Please lower the sectioning speed. • The sectioning thickness is too high: Please lower the thickness. • The clearance angle is too wide: Please try systematically to reduce the clearance angle settings until an optimum clearance angle has been found.

• The clamping of the specimen or knife holder might be insufficient: Please check all screws and clamping mechanisms at the specimen holder system and knife holder and retighten, if necessary. • The type of knife is inappropriate: Try and use a more stable knife (e.g. D-profile knife). • The material to be sectioned might be too hard: Please soften the block by soaking it in iced water or softening agent.

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