Stress control of the coating is it! Soft cantilevers would easily bend by coating. If you have not any experience with the coating, you should choose stiffer cantilevers to start with.
Of course, you should take good care to the tip sharpness. Good luck in your lab.! • Which type of the cantilever is suitable for AC mode AFM in water? For AC mode AFM operation in water, the short cantilever (100um long) in OMCL-TR400PSA- is recommended.
The customer who has successfully taken a DNA image in air with OMCL-AC120TS- (right image in left), must have confidence in oneself on the sample treatment. Then try it in water with OMCL-TR400PSA-! Both images (left) are taken by OLYMPUS NVB100 (SPM for biology).
OMCL-TR400PSA- has two types of cantilevers, 100 um long and 200 um long. The resonant frequencies of those cantilevers shift lower to about 7 kHz and 3 kHz in water respectively however they are 34 kHz and 11 kHz in air. Because 3 kHz is low, it must be close to the resonant frequency of ... more.
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