Why does DNA polymerase work in direction 5' to 3' not from 3' to 5'?

Because DNA Polymerase requires the OH on the 3' as an active site. It uses the OH on the 3' end of a nucleotide to attach a phosphate from the 5' end of the next nucleotide. It only works in this direction, and that is why DNA polymerase works 5' to 3'.

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