How does SABiosciences control the quality of the RT² qPCR Primer Assays?

Each RT² PCR Primer Assay is validated at SABiosciences, with both a real-time and conventional PCR quality control assay. These assays are carried out using a single source of genomic DNA. In order to pass QC, each primer assay must generate a single band of the correct predicted size by agarose gel electrophoresis and a single peak in the real-time dissociation curve without the appearance of primer dimers.

The amplification efficiencies (DART method) and sensitivities of each primer set are also validated. More.

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