For antibody-mediated direct detection, use of 10–50 ng labeled antibody per assay point is recommended. For indirect detection, 10 ng primary antibody and 50–200 ng labeled secondary antibody per ass... more.
I cant really gove you an answer,but what I can give you is a way to a solution, that is you have to find the anglde that you relate to or peaks your interest. A good paper is one that people get drawn into because it reaches them ln some way.As for me WW11 to me, I think of the holocaust and the effect it had on the survivors, their families and those who stood by and did nothing until it was too late.