Although Nagios was one of options that we've considered, we've chosen OpenNMS for monitoring purposes. Rationale for our decision is that OpenNMS is highly reliable and configurable free open-source tool and additionally, most of our applications are Java-based; OpenNMS offers integration with JMX. However, bear in mind that if you're demanding very complex tests for your Web site maybe it's better to look elsewhere.
OpenNMS can be set to check for HTTP status codes etc., but if you're looking for complex scenarios take a look at.
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