Redeploying an ASP.NET site in IIS7 without files in use interfering?

What you need to do is temporary stop IIS from processing any incoming requests for that app, so you can copy the new files and then start it again. This will lead to a small downtime for your clients, but unless your website is mission critical, that shouldn't be that big of a problem.

Another solution is IIS Programmatic Administration.

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