Why are frosted plates not recommended for QPCR?

Frosted plates have an uneven surface, causing uneven refraction of light and therefore high well-to-well variance. As consistency is a prerequisite to accuracy, variance should be kept to a minimum. Therefore frosted consumables are not recommended for QPCR.

More.

I cant really gove you an answer,but what I can give you is a way to a solution, that is you have to find the anglde that you relate to or peaks your interest. A good paper is one that people get drawn into because it reaches them ln some way.As for me WW11 to me, I think of the holocaust and the effect it had on the survivors, their families and those who stood by and did nothing until it was too late.