How can I calculate as exactly as possible the factor for the photometric determination of concentration of oligonucleotides?

In general, the following applies: F = 1: (e x l) where F = factor (µg/ml)-1 cm-1, e = molar extinction coefficient M-1 cm-1, l = optical layer thickness of cuvette cm. For small molecules like oligonucleotides, for example, the correct extinction coefficient is determined from the base composition. As the concentration of oligonucleotides is commonly reported as mmol/liter, a millimolar extinction coefficient (E) is conventionally used in the Beer-Lambert equation by means of: E = A (15.3) + G (11.9) + C (7.9) + T (9.3) A, G, C and T here stand for the number of corresponding bases in this oligonucleotide, the numbers in brackets for the molar extinction coefficient of any deoxynucleotide at pH 7.0.

Source: Sambrook et. Al. Molecular Cloning (2001).

Third Edition, A8. More.

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