Why ASCx disappear if clicking button to Save?

If you add controls programmatically in code, every post-back operation needs to re-create the same controls (e.g. During Page_Load) to process the post-back event (in your case, the Save button).

Dynamically added controls have no object reference variable in the codebehind class. They appear only in the control collection of the containing control, i.e. The Page.

Controls collection. When the page is posted back to the server as a result of user interaction a new instance of the codebehind class is instantiated, and all the variables of the class is set with values from the ViewState. See here.

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