Can I use a single file as a buffer? I.e. write to and read from at same time?

Most systems has several solutions for what you want to do, such as pipes and unix sockets. These are intended for this, unlike regular files. There are however programs that does this on regular files, and I think the clearest example of this is the unix-utility tail, which can "follow" a file.

You can use memory-mapped files, standard Python module called mmap.

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